Involvement of Aquaporin 1 in the Motility and in the Production of Fibrillin 1 and Type I Collagen of Cultured Human Dermal Fibroblasts

Aminocarbonyl proteins increase with age in the dermal layer. Gene Chip analysis of mRNA expression human fibroblasts cultured on collagen gels treated glyceraldehyde as an aminocarbonyl protein and untreated showed a decrease amount aquaporin 1 (AQP1) mRNA. In this study, we clarified involvement AQP1 gel contraction production fibrillin type I fibroblasts. experiment, siRNA was transfected into to deplete AQP1, cell motility contractile activity were assessed. The also examined by RT–qPCR antibody staining. depletion decreased activity, both staining decreased. Furthermore, reduced levels F-actin phosphorylated myosin light chain 2, suggesting their reductions These findings suggest that is important biomolecule for results extracellular matrix such collagen.